DNAmend 1.01 - a program for creating virtual DNA constructs
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Features

DNAmend is cloning software. That means, it may serve lab-workers
in molecular biology and genetic engineering to monitor the DNA-cloning 
process. We tried to create a PC routine, that provides on-line control on 
some steps of a cloning protocol - including the selection of the vectors 
and fragments, their processing by restrictase digestion, subsequent 
ligation, and the analysis of the cloned DNA.
The software is designed to accept files containing DNA sequence 
information in any file format based on PC-text. 
A set of pull-down menu commands enables the user to change the  mode of
viewing the DNA-sequences, print them, and above all manipulate them. 
You can search DNA-sequences for restriction sites, open reading frames 
and certain sequence patterns, translate the sequences and simulate 
Restriction, Ligation or End modification on-line. 
You may export maps of your DNA-sequences via clipboard into other windows
applications to prepare them for publication. 

The program runs on IBM-compatibles (at least 368, 4 MB RAM) under 
Microsoft Windows 3.x, Windows 95, and Windows NT and contains a detailed
on-line help.


Installation


The archive dnam101e.zip contains the following files:
		
		dnamende.exe		
		dnamende.hlp
		enzdat.dll
		example.squ
		order.txt
		license.txt
		whatsnew.txt
		readme.txt 		(this file) 

The file order.txt contains information on how to purchase the full version.

Unzip the dnam101e.zip to a new directory on your hard disk (e. g. c:\dnamend\).
Drag and drop dnamende.exe to your desktop. 
Run the program by doubleclicking the icon. 

Open the file "example.squ" to get an impression of the features of 
DNAmend. Make the window full size. You may search the restriction sites 
of EcoRV in the plasmid pEX2 and cut the plasmid with that enzyme. 
Religate two of the new fragments to new circular DNA.


Note:
If you do not have ctl3d.dll, get it for better graphical representation.
You must copy it to your windows-directory (e.g. c:\windows\system\). 
However, this file is absolutely dispensible for a full function of 
DNAmend!


Thank you for testing DNAmend!

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